Dual-modality endomicroscopy with co-registered fluorescence and phase contrast

Dual-modality endomicroscopy with co-registered fluorescence and phase contrast.

We describe a dual-modality laser scanning endomicroscope that provides simultaneous fluorescence contrast based on confocal laser endomicroscopy (CLE) and phase-gradient contrast based on scanning oblique back-scattering microscopy (sOBM). The probe consists of a 2.6mm-diameter micro-objective attached to a 30,000-core flexible fiber bundle. The dual contrasts are inherently co-registered, pro...

Fluorescence endomicroscopy with structured illumination.

We present an endomicroscope apparatus that utilizes structured illumination to produce high resolution (approximately 2.6 microm) optically sectioned fluorescence images over a field of view of about 240 microm. The endomicroscope is based on the use of a flexible imaging fiber bundle with a miniaturized objective. We also present a strategy to largely suppress structured illumination artifact...

Registration of Confocal Fluorescence Endomicroscopy Images Using Phase Correlation

The emerging confocal fluorescence endomicroscope is capable of imaging living tissues in a non-invasive way using a probe to continuously scan the surface and subsurface tissue structures. Due to possible tissue movement and tissue expansion/contraction, the acquired images contain various noises and distortions. It is necessary to align these images in order to obtain a better 3D reconstructi...

Two-photon Fluorescence Endomicroscopy

Co-registered MR tissue phase mapping and speckle tracking echocardiography: inter-modality comparison of regional myocardial velocities in pediatric patients

Methods N=19 patients with congenital heart abnormalities and biventricular physiology who had undergone cardiac MRI including TPM and transthoracic echocardiography within 6 months of MRI were identified (mean age=14 (7-20) years). Average time between MR and echo studies was 2.3±1.4 months. MRI was performed on a 1.5T MR-system (Aera, Siemens, Erlangen, Germany). TPM were acquired data in sho...